M. And Feneley, M. P. In both plant and cell cultures GST catalysed conjugation of the diphenyl ethers acifluorfen and fomesafen was over five fold greater in the presence of homoglutathione as compared with glutathione. The preferential detoxification of these herbicides in the presence of homoglutathione appeared to be an important determinant of their rapid detoxification in soybean and an important factor in herbicide selectivity. GSTs were purified from five day old soybean cell cultures using S hexylglutathione affinity chromatography and anion exchange chromatography.
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